Titre : | The quality of DNA extracted from liquid or dried blood is not adversely affected by storage at 4°C for up to 24 h. (2008) |
Auteurs : | HALSALL (Antony) : GBR. Manchester Interdisciplinary Biocentre. The University of Manchester. Manchester. ; DAVIDSON (Alice) : GBR. Centre for Molecular Medicine. The University of Manchester Stopford Building. ; Rupert GAUT ; Stephen LITTLE ; Paul RAVETTO ; Yancy REYES ; Nicola THELWELL ; Dxs Ltd. Manchester. GBR |
Type de document : | Article |
Dans : | International journal of epidemiology (vol. 37, 2008) |
Pagination : | I7-I10 |
Langues: | Anglais |
Mots-clés : | Qualité ; ADN ; Sang ; Conservation ; Banque tissu ; Génétique |
Résumé : | [BDSP. Notice produite par INIST-CNRS rDR0x9mp. Diffusion soumise à autorisation]. Background : A consistent and stable source of DNA is an essential requirement for many Biobanks. An important pre-analytical variable is the delay between sample collection and sample processing. Methods : Fresh blood samples (n=80) were collected and either processed immediately or after storage for 24 h. The samples were either stored as liquid blood at 4°C or as dried blood spots at ambient temperature on three types of paper-based storage media. The quality of the DNA extracted from the samples was measured. Results : No difference was observed between fresh and stored blood samples. Conclusions : The quality of DNA extracted from liquid or dried blood is not adversely affected by storage at 4°C for up to 24 h. |