Titre :
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Associations with early-life socio-economic position in adult DNA methylation. (2012)
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Auteurs :
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Nada BORGHOL ;
Michael HALLETT ;
Clyde HERTZMAN ;
Wendy MCARDLE ;
Marcus PEMBREY ;
Chris POWER ;
Ariane RACINE ;
Matthew SUDERMAN ;
Moshe SZYF ;
Department of Pharmacology and Therapeutics. McGill University. Montreal. PQ. CAN ;
McGill Centre for Bioinformatics. McGill University. Montreal. PQ. CAN ;
Sackler Program for Epigenetics & Developmental Psychobiology. McGill University. Montreal. PQ. CAN
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Type de document :
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Article
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Dans :
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International journal of epidemiology (vol. 41, n° 1, 2012)
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Pagination :
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62-74
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Langues:
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Anglais
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Mots-clés :
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Association
;
Facteur socioéconomique
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Adulte
;
ADN
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Grande Bretagne
;
Enfant
;
Homme
;
Europe
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Résumé :
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[BDSP. Notice produite par INIST-CNRS F9qR0xC7. Diffusion soumise à autorisation]. Background Disadvantaged socio-economic position (SEP) in childhood is associated with increased adult mortality and morbidity. We aimed to establish whether childhood SEP was associated with differential methylation of adult DNA. Methods Forty adult males from the 1958 British Birth Cohort Study were selected from SEP extremes in both early childhood and mid-adulthood. We performed genome-wide methylation analysis on blood DNA taken at 45 years using MeDIP (methylated DNA immunoprecipitation). We mapped in triplicate the methylation state of promoters of approximately 20000 genes and 400 microRNAs. Probe methylation scores were averaged across triplicates and differential methylation between groups of individuals was determined. Differentially methylated promoter sites of selected genes were validated using pyrosequencing of bisulfite-converted DNA. Results Variably methylated probes (9112 from n=223 359 on the micro-array) corresponded to 6176 gene promoters with at least one variable probe. Unsupervised hierarchical clustering of probes obtained from the 500 most variable promoters revealed a cluster enriched with high SEP individuals confirming that SEP differences contribute to overall epigenetic variation. Methylation levels for 1252 gene promoters were associated with childhood SEP vs 545 promoters for adulthood SEP. Functionally, associations with childhood SEP appear in promoters of genes enriched in key cell signalling pathways. The differentially methylated promoters associated with SEP cluster in megabase-sized regions of the genome. Conclusions Adult blood DNA methylation profiles show more associations with childhood SEP than adult SEP. Organization of these associations across the genome suggests a well-defined epigenetic pattern linked to early socio-economic environment.
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