Titre :
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Public Health Laboratories. Pandemic Preparedness in Hawaii : A Multicenter Verification of Real-Time RT-PCR for the Direct Detection of Influenza Virus Types A and B. (2010)
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Auteurs :
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A. CHRISTIAN WHELEN ;
Matthew-J BANKOWSKI ;
Amelia CHAN ;
Paul-V EFFLER ;
. FRANCES POUCH DOWNES, éd. ;
Glenn FURUYA ;
Karen HIGA ;
Stacey HONDA ;
Terrie KOYAMATSU ;
Diane KUMASHIRO ;
Roland LEE ;
Nathaniel MOORE ;
John-C RIDDERHOF, éd. ;
Robert UEKI ;
Clinical Laboratories of Hawaii. Ewa Beach. HI. USA ;
Diagnostic Laboratory Services and The Queens Medical Center. Honolulu. HI. USA ;
John A Burns School of Medicine. University of Hawaii. Honolulu. HI. USA ;
Kaiser-Permanente. Honolulu. HI. USA ;
State Laboratories Division. Hawaii State Department of Health. Pearl City. HI. USA
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Type de document :
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Article
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Dans :
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Public health reports (vol. 125, 2010)
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Pagination :
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81-87
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Langues:
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Anglais
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Mots-clés :
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Monde
;
Enseignement
;
Polynésie
;
Océanie
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Virus
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Résumé :
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[BDSP. Notice produite par INIST-CNRS R0x9AnpC. Diffusion soumise à autorisation]. Objective. We integrated multicenter, real-time (RTi) reverse transcription polymerase chain reaction (RT-PCR) screening into a statewide laboratory algorithm for influenza surveillance and response. Methods. Each of three sites developed its own testing strategy and was challenged with one randomized and blinded panel of 50 specimens previously tested for respiratory viruses. Following testing, each participating laboratory reported its results to the Hawaii State Department of Health, State Laboratories Division for evaluation and possible discrepant analysis. Results. Two of three laboratories reported a 100% sensitivity and specificity, resulting in a 100% positive predictive value and a 100% negative predictive value (NPV) for influenza type A. The third laboratory showed a 71% sensitivity for influenza type A (83% NPV) with 100% specificity. All three laboratories were 100% sensitive and specific for the detection of influenza type B. Discrepant analysis indicated that the lack of sensitivity experienced by the third laboratory may have been due to the analyte-specific reagent probe used by that laboratory. Use of a newer version of the product with a secondary panel of 20 specimens resulted in a sensitivity and specificity of 100%. Conclusions. All three laboratories successfully verified their ability to conduct clinical testing for influenza using diverse nucleic acid extraction and RTi RT-PCR platforms. Successful completion of the verification by all collaborating laboratories paved the way for the integration of those facilities into a state-wide laboratory algorithm for influenza surveillance and response.
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