Titre :
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EBV Immortalization of human B lymphocytes separated from small volumes of cryo-preserved whole blood. (2008)
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Auteurs :
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M.M. AMOLI ;
D. CARTHY ;
W.E.R. OLLIER ;
H. PLATT ;
Centre for Integrated Genomic Medical Research. University of Manchester. Manchester. GBR
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Type de document :
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Article
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Dans :
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International journal of epidemiology (vol. 37, 2008)
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Pagination :
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I41-I45
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Langues:
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Anglais
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Mots-clés :
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Homme
;
Banque tissu
;
Génétique
;
Epidémiologie
;
Virus
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Résumé :
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[BDSP. Notice produite par INIST-CNRS s7osrR0x. Diffusion soumise à autorisation]. EBV immortalized B lymphocyte cell lines have been extensively used as a source of biological material for functional and molecular studies and represent a potentially limitless source of genomic DNA. Current technologies for EBV transformation are costly and use relatively large volumes of peripheral blood. Alternative methods were examined to determine whether smaller volumes of cryo-preserved whole blood could be subsequently transformed and which could provide a more cost-effective strategy for large population-based studies such as UK Biobank. A successful method was established where viable B cells were positively selected from 0.5ml cryo-preserved whole blood samples. These were EBV transformed in microtitre plates and subsequently expanded in culture. A pilot study within UK Biobank was performed, which confirmed its potential usefulness for this study.
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