| Titre : | Effects of the UK Biobank collection protocol on potential biomarkers in saliva. (2012) |
| Auteurs : | PRAMANIK (Rashida) : GBR. Nihr Biomedical Research Centre. Guy's & St Thomas'Hospital Nhs Foundation Trust. London. ; GALLOWAY (John) : GBR. Dental Teams Study Unit. Joint Ucl. Uclh Biomedical Research Centre. University College London Hospital Nhs Foundation Trust. London. ; James-O KISTLER ; PEAKMAN (Tim) : GBR. Uk Biobank. Stockport. ; Gordon-B PROCTOR ; Hayley THOMPSON ; William-G WADE ; Microbiology Unit. Department of Clinical and Diagnostic Sciences. King's College London Dental Institute. London. GBR ; Salivary Research Unit. Department of Clinical and Diagnostic Sciences. King's College London Dental Institute. London. GBR |
| Type de document : | Article |
| Dans : | International journal of epidemiology (vol. 41, n° 6, 2012) |
| Pagination : | 1786-1797 |
| Langues: | Anglais |
| Mots-clés : | Banque tissu ; Salive ; Voie orale ; Diagnostic ; Hormone ; ADN ; ARN ; Europe |
| Résumé : | [BDSP. Notice produite par INIST-CNRS R0xDln8p. Diffusion soumise à autorisation]. Background The UK Biobank (UKB) is a national epidemiological study of the health of 500000 people, aged 40-69 years, who completed health-related tests and a questionnaire and gave samples of blood and urine. Salivas collected from 120000 of these subjects were transported at 4° C and were placed in ultra-low temperature archives at up to 24 h after collection. The present study assessed how changes in saliva composition under UKB conditions influence a range of potential biomarkers resulting from holding saliva at 4° C for 24 h. Methods Unstimulated whole-mouth saliva samples were collected from 23 volunteers aged 45-69 years. Salivas were split into aliquots some of which were immediately frozen at - 80° C, whereas others were stored at 4° C for 24 h and then frozen at - 80° C, mimicking the UKB protocol. Results Assessment of mRNA by real-time polymerase chain reaction revealed no difference between samples that were analysed after the UKB protocol and those that were immediately preserved. Immunochemical analysis showed some loss of bêta-Actin under UKB conditions, whereas other salivary proteins including cytokines and C-reactive protein appeared to be unaffected. Cortisol and showed no reduction by UKB conditions, but salivary nitrite was reduced by 30%. The oral microbiome, as revealed by sequencing 16S rRNA genes, showed variations between subjects, but paired samples within subjects were very similar. Conclusions Our results suggest that many salivary components remain little affected under UKB collection and handling protocols, suggesting that the resource of 120000 samples held in storage will be useful for phenotyping subjects and revealing potential prognostic disease biomarkers. |
Service Documentation EHESP
Me connecter
PORTAIL DOCUMENTAIRE
École des hautes études en santé publique
Catalogue
Multi-bases
Accueil
Sélection de la langue
Adresse
Service Documentation EHESPBibliothèque Rennes Avenue du Professeur Léon Bernard – CS 74312 – 35043 Rennes Cedex Bibliothèque MSSH Paris 20 avenue George Sand - 93210 La Plaine Saint-Denis
France
Bibliothèque Rennes : 02 99 02 29 50 Bibliothèque MSSH Paris : 02 99 02 24 50
contact