| Titre : | Predictors of global methylation levels in blood DNA of healthy subjects : a combined analysis. (2012) |
| Auteurs : | Zhong-Zheng ZHU ; Pietro APOSTOLI ; Andrea BACCARELLI ; Pier-Alberto BERTAZZI ; Valentina BOLLATI ; Matteo Bonzini ; Laura CANTONE ; Wong-Ho CHOW ; Giovanni COSTA ; Silvia FUSTINONI ; . LIFANG HOU ; Jolanta LISSOWSKA ; Barbara MARINELLI ; Angela-C PESATORI ; Joel SCHWARTZ ; Letizia TARANTINI ; Pantel VOKONAS ; Allen-S YANG ; Center of Molecular and Genetic Epidemiology. Department of Environmental and Occupational Health. Università degli Studi di Milano and Irccs Maggiore Policlinico Hospital. Mangiagalli and Regina Elena Foundation. Milan. ITA ; Exposure Epidemiology and Risk Program. Department of Environmental Health. Harvard School of Public Health. Boston. MA. USA |
| Type de document : | Article |
| Dans : | International journal of epidemiology (vol. 41, n° 1, 2012) |
| Pagination : | 126-139 |
| Langues: | Anglais |
| Mots-clés : | Sang ; ADN |
| Résumé : | [BDSP. Notice produite par INIST-CNRS oR0x8AD8. Diffusion soumise à autorisation]. Background Estimates of global DNA methylation from repetitive DNA elements, such as Alu and LINE-1, have been increasingly used in epidemiological investigations because of their relative low-cost, high-throughput and quantitative results. Nevertheless, determinants of these methylation measures in healthy individuals are still largely unknown. The aim of this study was to examine whether age, gender, smoking habits, alcohol drinking and body mass index (BMI) are associated with Alu or LINE-1 methylation levels in blood leucocyte DNA of healthy individuals. Methods Individual data from five studies including a total of 1465 healthy subjects were combined. DNA methylation was quantified by PCR-pyrosequencing. Results Age [bêta=-0.011% of 5-methyl-cytosine (% 5mC)/year, 95% confidence interval (CI) - 0.020 to - 0.001% 5mC/year] and alcohol drinking (bêta=-0.214,95% CI - 0.415 to - 0.013) were inversely associated with Alu methylation. Compared with females, males had lower Alu methylation (bêta=-0.385,95% CI - 0.665 to - 0.104) and higher LINE-1 methylation (bêta=0.796,95% CI 0.261 to 1.330). No associations were found with smoking or BMI. Percent neutrophils and lymphocytes in blood counts exhibited a positive (bêta=0.036,95% CI 0.010 to 0.061) and negative (bêta=-0.038,95% CI - 0.065 to - 0.012) association with LINE-1 methylation, respectively. Conclusions Global methylation measures in blood DNA vary in relation with certain host and lifestyle characteristics, including age, gender, alcohol drinking and white blood cell counts. These findings need to be considered in designing epidemiological investigations aimed at identifying associations between DNA methylation and health outcomes. |
Service Documentation EHESP
Me connecter
PORTAIL DOCUMENTAIRE
École des hautes études en santé publique
Catalogue
Multi-bases
Accueil
Sélection de la langue
Adresse
Service Documentation EHESPBibliothèque Rennes Avenue du Professeur Léon Bernard – CS 74312 – 35043 Rennes Cedex Bibliothèque MSSH Paris 20 avenue George Sand - 93210 La Plaine Saint-Denis
France
Bibliothèque Rennes : 02 99 02 29 50 Bibliothèque MSSH Paris : 02 99 02 24 50
contact