| Titre : | Relative rates of non-pneumonic SARS coronavirus infection and SARS coronavirus pneumonia. (2004) |
| Auteurs : | Patrick-Cy WOO ; Kwok-Hung CHAN ; Xiao-Yan Che ; Vincent-Cc CHENG ; Ivan-Fn HUNG ; Susanna-Kp LAU ; Victoria-Kp TAM ; Sidnev-Cf TAM ; Hoi-Wah TSOI ; Beatrice-Hl Wong ; Samson-Sy WONG ; . YI GUAN ; Kwok-Yung YUEN ; Bo-Jian ZHENG ; Queen Mary Hospital. University of Hong Kong. Department of Microblology. HKG |
| Type de document : | Article |
| Dans : | Lancet (The) (vol. 363, n° 9412, 2004) |
| Pagination : | 841-845 |
| Langues: | Anglais |
| Mots-clés : | Infection ; Taux ; Virus ; Pneumonie ; Epidémiologie ; Epidémie ; Hongkong ; Chine ; Asie ; Virose ; Génome ; Homme ; Appareil respiratoire [pathologie] |
| Résumé : | [BDSP. Notice produite par INIST-CNRS R3hR0xqk. Diffusion soumise à autorisation]. Background : Although the genome of severe acute respiratory syndrome coronavirus (SARS-CoV) has been sequenced and a possible animal reservoir identified, seroprevalence studies and mass screening for detection of subclinical and non-pneumonic infections are still lacking. Methods : We cloned and purified the nucleocapsid protein and spike polypeptide of SARS-CoV and examined their immunogenicity with serum from patients with SARS-CoV pneumonia. An ELISA based on recombinant nucleocapsid protein for IgG detection was tested with serum from 149 healthy blood donors who donated 3 years previously and with serum positive for antibodies against SARS-CoV (by indirect immunofluorescence assay) from 106 patients with SARS-CoV pneumonia. The seroprevalence of SARS-CoV was studied with the ELISA in healthy blood donors who donated during the SARS outbreak in Hong Kong, non-pneumonic hospital inpatients, and symptom-free health-care workers. All positive samples were confirmed by two separate western-blot assays (with recombinant nucleocapsid protein and recombinant spike polypeptide). Findings : Western-blot analysis showed that the nucleocapsid protein and spike polypeptide of SARS-CoV are highly immunogenic. The specificity of the IgG antibody test (ELISA with positive samples confirmed by the two western-blot assays) was 100%, and the sensitivity was 94.3%. Three of 400 healthy blood donors who donated during the SARS outbreak and one of 131 non-pneumonic paediatric inpatients were positive for IgG antibodies, confirmed by the two western-blot assays (total, 0.48% of our study population). Interpretation Our findings support the existence of subclinical or non-pneumonic SARS-CoV infections. Such infections are more common than SARS-CoV pneumonia in our locality. |
Service Documentation EHESP
Me connecter
PORTAIL DOCUMENTAIRE
École des hautes études en santé publique
Catalogue
Multi-bases
Accueil
Sélection de la langue
Adresse
Service Documentation EHESPBibliothèque Rennes Avenue du Professeur Léon Bernard – CS 74312 – 35043 Rennes Cedex Bibliothèque MSSH Paris 20 avenue George Sand - 93210 La Plaine Saint-Denis
France
Bibliothèque Rennes : 02 99 02 29 50 Bibliothèque MSSH Paris : 02 99 02 24 50
contact