Titre : | Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from water samples. (2002) |
Auteurs : | P. KARANIS ; A. KIMURA |
Type de document : | Article |
Dans : | Letters in applied microbiology (vol. 34, n° 6, 2002) |
Pagination : | 444-449 |
Langues: | Anglais |
Mots-clés : | Méthode ; Protozoaire ; Agent pathogène ; Eau consommation humaine |
Résumé : | [BDSP. Notice produite par INIST-CNRS n6hjR0x0. Diffusion soumise à autorisation]. Aims : Evaluation of three flocculation methods for the purification of Cryptosporidium parvum oocysts from tap water. Methods and Results : Ferric sulphate, aluminium sulphate and calcium carbonate were compared for their recovery efficiency of C. parvum oocysts from tap water. Lower mean recovery was achieved by calcium carbonate (38.8%) compared with ferric sulphate (61.5%) and aluminium sulphate (58.1%) for the recovery of 2.5 x 105 oocysts l-1 ; 2.5 oocysts l-1 and 1 oocyst l-1 were adequately purified using ferric sulphate flocculation. In vitro excystation experiments showed that ferric sulphate flocculation does not markedly reduce the viability of oocysts. Conclusions : Ferric sulphate flocculation is a simple and effective tool for the purification of C. parvum oocysts from tap water. Significance and Impact of the Study : The high recovery rates and low impact on oocyst viability provided by ferric sulphate flocculation might be useful for the detection of Cryptosporidium oocysts in environmental water samples. |