| Titre : | Self-collection of oral epithelial cell DNA under instruction from epidemiologic interviewers. (2000) |
| Auteurs : | Lea-C HARTY ; Neil-E CAPORASO ; Richard-B HAYES ; Peter-G SHIELDS ; Deborah-M WINN ; Genetic Epidemiology Branch. National Cancer Institute. Bethesda. MD. USA |
| Type de document : | Article |
| Dans : | American journal of epidemiology (vol. 151, n° 2, 2000) |
| Pagination : | 199-205 |
| Langues: | Anglais |
| Mots-clés : | Cancer ; Cavité buccale ; Homme |
| Résumé : | [BDSP. Notice produite par INIST v52R0xoH. Diffusion soumise à autorisation]. Oral epithelial cells provide an easily accessible source of germline DNA. Two methods for collection were compared in a 1992-1995 case-control study of oral cancer in Puerto Rico. One group of subjects (55 controls without oral cancer) collected oral rinse samples at home or work under the direction of a nonmedically trained interviewer ("self-collection") ; the other group (94 controls) participated in a clinic-based collection, which also included blood and urine samples, conducted by a medical technician ("clinic collection"). Participation was higher for self-collection (98.2%) than for clinic collection (70.7%) (p<0.001). DNA yields ranged from 2.0 to 204.5 mug (median, 25.9 mug) and did not differ by collection method, although yields varied by interviewer among self-collected samples (p=0.02). Success rates for polymerase chain reaction amplification of the ADH3, NAT1, and multiplex CYP1A1/GSTT1/GSTM1 genotyping assays ranged from 76.4% (NAT1) to 98.2% (ADH3) for self-collected samples and were similar to those for clinic-collected samples (87.2-97.9%). Failure to amplify was associated with low DNA content (p=0.015). Similar results were observed among cases (91 self-collected, 66 clinic collected), except that DNA yields did not vary by interviewer and a larger fraction (10.2%) of samples contained less than 5 mug of DNA, perhaps because of disease-related oral impairment. (...) |
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